human mirna microarray kit (v2  (Agilent technologies)

Journal: BioMed Research International

Article Title: Integration Analysis of MicroRNA and mRNA Expression Profiles in Human Peripheral Blood Lymphocytes Cultured in Modeled Microgravity

doi: 10.1155/2014/296747

Figure Lengend Snippet: Differentially expressed miRNAs in human PBLs incubated in MMG. (a) The expression level of each miRNA, indicated as fold change, is the mean of the expression values obtained from the transformed log2 ratio (MMG/1 g). (b) Dendrogram of miRNAs differentially expressed in MMG. The range of expression value is from −3.7 (green, downregulation) to 3.07 (red, upregulation). Grey boxes correspond to not available (N/A) fluorescent signal from the microarray platform.

Article Snippet: MicroRNAs profiling was carried out in PBL samples incubated in MMG versus 1 g. Analyses were performed by using the “Human miRNA Microarray kit (V2)” (Agilent Technologies) that allows the detection of 723 known human (miRBase v.10.1) and 76 human viral miRNAs.

Techniques: Incubation, Expressing, Transformation Assay, Microarray

Journal: BioMed Research International

Article Title: Integration Analysis of MicroRNA and mRNA Expression Profiles in Human Peripheral Blood Lymphocytes Cultured in Modeled Microgravity

doi: 10.1155/2014/296747

Figure Lengend Snippet: Microarray data validation by quantitative real-time PCR (qRT-PCR). Validation of microarray data by qRT-PCR in MMG-incubated versus 1 g incubated PBLs. The results are consistent with the cumulative microarray data of miRNAs (a) and mRNAs (b). Values (fold change, dark grey bars) are means ± S.E. of expression levels calculated as the log2 (MMG/1 g) on PBL samples from 4 to 6 different donors. The value “1” of control 1 g PBLs (light grey bars) is arbitrarily given when no change is observed (*** P < 0.001, ** P < 0.01, and * P < 0.05, t -test).

Article Snippet: MicroRNAs profiling was carried out in PBL samples incubated in MMG versus 1 g. Analyses were performed by using the “Human miRNA Microarray kit (V2)” (Agilent Technologies) that allows the detection of 723 known human (miRBase v.10.1) and 76 human viral miRNAs.

Techniques: Microarray, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Incubation, Expressing

Journal: BMC Genomics

Article Title: Pediatric primary central nervous system germ cell tumors of different prognosis groups show characteristic miRNome traits and chromosome copy number variations

doi: 10.1186/1471-2164-11-132

Figure Lengend Snippet: Gene expression analysis of the different GCT subgroups . ( A ) A multidimensional scaling (MDS) plot containing the differentially expressed genes (690 probe sets, q < 0.001). Each spot represents a single array. ( B ) A comparison of the transcriptome traits between ESCs and NGMGCTs by principal component analysis (PCA). ( C ) Relationships between ESCs, germinomas and NGMGCTs. Average linkage Euclidean distances between the tissues and ESC were calculated using genes distinguishing the filtrated 690-probe set. The confidence limits shown represent the standard error. ( D ) A heat map shows genes enriched in the ESCs and in the different prognostic groups ( q < 0.001). ( E-F ) Real-time PCR validation of the microarray data. Mean expression levels of the examined genes were compared to that of the GAPDH control. Each bar represents a different individual ( F ). The genes' array hybridization signals are also shown ( E ).

Article Snippet: The Agilent Human miRNA Microarray Kit V2 (Agilent, Foster City, CA, USA) containing probes for 723 human microRNAs from the Sanger database v10.1 was used.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Microarray, Hybridization